Factor II (Prothrombin 20210 G>A)
Test Code
Factor II (Prothrombin), FII
CPT Codes
83891-3G x1; 83892-3G x2; 83900-3G x1; 83914-3G x1; 83909-3G x1; 83912-3G x1; 83912-25 x1
Specimen
Whole blood or buccal swabs
Volume
5 mL of whole blood or four buccal swabs
Minimum Volume
3 mL of whole blood or four buccal swabs
Container
Lavender-stopper (EDTA) tube or paper envelope for dried buccal swabs
Storage Instructions
Maintain at room temperature or refrigerate
Cause for Rejection
Hemolyzed specimen; quantity not sufficient
Use
The Factor II (prothrombin) polymorphism is the result of a single point mutation (20210 G>A) in the 3’ untranslated region of the gene. The 20210 G>A gives rise to increased circulating prothrombin levels, thus creating the potential of a hypercoagulable state. This polymorphism is found in approximately 2% of individuals in the U.S. and it raises the risk of thrombosis significantly for both males and females in all age groups. People with this polymorphism are at 2-3 fold increased risk of deep venous thrombosis (DVT), without other confounding non-genetic factors such as smoking, hormone therapy, and immobility.
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Limitations
Other genetic variants of the Factor II gene that are not detected in the assay may influence Factor II enzymatic activity. Other genetic and non-genetic factors may also influence the balance of proper coagulation.
Methodology
Multiplex polymerase chain reaction and allele specific primer extension (Tag-It [TM]).
Turnaround Time
Five business days after receipt of specimen. STAT turnaround time of 3 business days is available for an additional charge.
