Factor II (Prothrombin 20210 G>A)
Factor II (Prothrombin), FII
Whole blood or buccal swabs
5 mL of whole blood or four buccal swabs
3 mL of whole blood or four buccal swabs
Lavender-stopper (EDTA) tube or paper envelope for dried buccal swabs
Maintain at room temperature or refrigerate
Cause for Rejection
Hemolyzed specimen; quantity not sufficient
General Purpose and Use
The Factor II (prothrombin) polymorphism is the result of a single point mutation (20210 G>A) in the 3’ untranslated region of the gene. The 20210 G>A gives rise to increased circulating prothrombin levels, thus creating the potential of a hypercoagulable state. This polymorphism is found in approximately 2% of individuals in the U.S. and it raises the risk of thrombosis significantly for both males and females in all age groups. People with this polymorphism are at 2-3 fold increased risk of deep venous thrombosis (DVT), without other confounding non-genetic factors such as smoking, hormone therapy, and immobility.
Other genetic variants of the Factor II gene that are not detected in the assay may influence Factor II enzymatic activity. Other genetic and non-genetic factors may also influence the balance of proper coagulation.
Real-time polymerase chain reaction with fluorescence detection.
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